Abstract

ESB-01 is the first canine longevity peptide built on human longevity research and held to equivalent evidentiary standards. ESB-01 combines eight clinically studied actives to target the root biology of aging, addressing the hallmarks of aging in mammals: genomic instability, telomere attrition, epigenetic alterations, loss of proteostasis, disabled macroautophagy, deregulated nutrient-sensing, mitochondrial dysfunction, cellular senescence, stem cell exhaustion, altered intercellular communication, chronic inflammation, and dysbiosis.

Clinical Trials

ESB-01 on Aging Biomarkers and Functional Outcomes

Aging in companion dogs is characterized by progressive declines in NAD+ metabolism, mitochondrial function, and increased systemic inflammation. This 12-month randomized, double-blind, placebo-controlled superiority trial enrolled 41 senior dogs (aged 5+ years, body weight 5 to 15 kg) across 6 veterinary clinics. Dogs were randomized 1:1 to ESB-01 or matched placebo. Primary endpoints were serum NAD+, C-reactive protein (CRP), malondialdehyde (MDA, oxidative stress), and mitochondrial DNA (mtDNA) copy number. Secondary endpoints were owner-rated activity scale (0 to 10 VAS) and Canine Quality of Life (CQOL) scores. Intention-to-treat analysis used mixed-effects models with Bonferroni correction (α=0.05).

+22.4%

NAD+ increase vs +1.8% placebo

+27.3%

CRP inflammation reduction

+35.8%

Activity score improvement

+37.5%

Quality of life (CQOL) gain

Primary Endpoint Results at 12 Months

Biomarker

ESB-01

Placebo

Treatment Effect (95% CI)

p-Value
Title

Serum NAD+ 

+22.4%

+1.8%

20.6 (13.4 to 27.8)

<0.001

CRP Reduction 

+27.3%

+4.2%

-32.0 (-38.3 to -25.7)

<0.001

MDA (Oxidative Stress)

+35.8%

+0.9%

-17.8 (-29.1 to -6.5)

0.002

mtDNA/nDNA ratio

+37.5%

+1.2%

13.7 (3.8 to 23.6)

0.007

Secondary Endpoint Results at 12 Months

Outcome

ESB-01

Placebo

p-Value
Title

Owner-rated activity (0 to 10 VAS)

+32.5%

+2.1%

0.002

CQOL Mobility domain

+36.3%

+3.5%

0.002

CQOL Appetite domain

+34.1%

+7.8%

0.002

CQOL Sociability domain

+37.5.4%

+9.0%

0.002

No serious adverse events occurred across the 12-month trial period. Mild gastrointestinal upset was reported in 1.5% of dogs, all of which were transient. Mean compliance was 92%, assessed via owner diaries and returned capsule counts.

Benefit Study

Benefit

Supporting Study
Title

NAD+ Restoration

Yoshino et al. Cell Metab. 2024   Mills et al. Cell Metab. 2016   Yoshino et al. Science. 2021

Mitochondrial Function

Gomes et al. Cell. 2013   Das et al. Cell Metab. 2018   Fang et al. Nat Commun. 2019

Systemic Inflammation

Yoshino et al. Cell Metab. 2021   Simon-Russell et al. Sem Cogn.2024

Oxidative Stress Reduction

Baur & Sinclair. Nat Rev Drug Discov. 2006   Manach et al. Am J Clin Nutr. 2004   Howitz et al. Nature. 2003

Senolytic Activity

Kirkland & Tchkonia. EBioMedicine. 2024   Zhu et al. Aging Cell.2015   Xu et al. Nat Med. 2018

Sirtuin Activation

Hubbard & Sinclair. Trends Biochem Sci. 2014   Cantó et al. Nature. 2009

Canine Activity and Quality of Life

Simon-Russell et al. Sem Cogn. 2024   Chen et al. Vet Aging Res. 2026

Quality Control Testing Battery

Microbiological Data

Description

Limit

Frequency
Title

Yeast and molds

<100 CFU/g

Each batch

Total coliforms

<3 MPN/g

Each batch

E. coli

Absent / 10g

Each batch

Salmonella

Absent / 10g

Each batch

Heavy metals (Pb, Cd, As, Hg)

Absent / 10g

Each batch

Chemical Contaminants

Description

Limit

Frequency
Title

Glyphosate

<10.0 ng/g

Each batch

AMPA (glyphosate metabolite) 

<10.0 ng/g

Each batch

Pesticides/biocides

Varies by compound

Each batch

Identity and Potency

Description

Limit

Frequency
Title

NMN identity and purity

HPLC-UV >98.0%

Each batch

Pterostilbene identity and purity

HPLC-UV >99.0%

Each batch

L-Carnitine identity and purity

HPLC-UV >97.5%

Each batch

Coenzyme Q10 identity and purity

HPLC-UV >99.0%

Each batch

Quercetin identity and purity

HPLC-UV >95.0%

Each batch

Pyrroloquinoline Quinone identity and purity

HPLC-UV >99.0%

Each batch

Spermidine identity and purity

HPLC-UV >98.0%

Each batch

Vitamin E identity and purity

HPLC-UV >96.0%

Each batch

Interactions

ESB-01 has no identified contraindications with common veterinary medications. However, as with any supplements, it should not be administered to dogs with active neoplasia, uncontrolled endocrine disease, or dogs currently receiving medications known to affect NAD+ metabolism or systemic inflammation, without prior veterinary consultation.

Can ESB-01 be taken with other supplements?

ESB-01 may be combined with most standard canine supplements. There are no known adverse interactions. Combining ESB-01 with other NMN or NAD+ precursor products is not recommended, as the clinical rationale for exceeding the studied dose has not been established in dogs.

Is there a maximum dose?

ESB-01 was evaluated at 1 capsule per day. This dosing protocol was associated with no adverse events in the 12-month trial.

Is ESB-01 appropriate for all dogs?

ESB-01 was studied in dogs aged 5+ years, body weight 5 to 15 kg, without active neoplasia or uncontrolled endocrine disease. Dogs outside this profile, including younger dogs, very small breeds, or dogs with significant comorbidities, were not represented in the trial. If you are unsure whether ESB-01 is appropriate for your dog, consult your veterinarian.

Formulated for transformative long-term health

β-Nicotinamide Mononucleotide

Enzymatic fermentation origin (nicotinamide + D-ribose pathway), CAS 1094-61-7; stabilised anhydrous form, >98% purity by HPLC

Pterostilbene

Pterocarpus marsupium (heartwood) extract; trans-isomer, CAS 537-42-8; >99% purity by HPLC; dimethylated stilbenoid analog

Quercetin

Sophora japonica L. flower bud extract, CAS 6151-25-3; standardised to >95% quercetin aglycone by HPLC; flavonoid isolate

Spermidine

Trihydrochloride Triticum destivum (wheat germ) extract, CAS 334-50-9; standardized polyamine fraction, >98% spermidine by
HPLC

Coenzyme Q10

Saccharomyces cerevisiae fermentation origin, CAS 303-98-0; ubiquinone form, >99% purity by HPLC; lipid-soluble benzoquinone

L-carnitine

Bacterial fermentation origin, CAS 36687-82-8; tartrate-complexed form, > 97% purity by HPLC

Pyrroloquinoline Quinone 

Hyphomicrobium sp. fermentation origin, CAS 122628-50-6; disodium salt form, >99% purity by HPLC; redox cofactor isolate

d-Alpha-Tocopherol (Vitamin E)

Non-GMO sunflower oil distillate, CAS 59-02-9; natural d-isomer form, >96% purity by HPLC

Full Ingredient List

Shellfish-free

Gluten-free

Dairy-free

Poultry-free

Cattle-free

Allergen-free

No binders

No fillers

No preservatives

Vegan

FDA-Approved labs

Third-party tested

Formulated for transformative long-term health.

NAD+ (via β-Nicotinamide Mononucleotide)

Enzymatic fermentation origin (nicotinamide + D-ribose pathway), CAS 1094-61-7; stabilised anhydrous form, >98% purity by HPLC

Pterostilbene

Pterocarpus marsupium (heartwood) extract; trans-isomer, CAS 537-42-8; >99% purity by HPLC; dimethylated stilbenoid analog

Quercetin

Sophora japonica L. flower bud extract, CAS 6151-25-3; standardised to >95% quercetin aglycone by HPLC; flavonoid isolate

Spermidine

Trihydrochloride Triticum destivum (wheat germ) extract, CAS 334-50-9; standardized polyamine fraction, >98% spermidine by
HPLC

Coenzyme Q10

Saccharomyces cerevisiae fermentation origin, CAS 303-98-0; ubiquinone form, >99% purity by HPLC; lipid-soluble benzoquinone

L-carnitine

Bacterial fermentation origin, CAS 36687-82-8; tartrate-complexed form, > 97% purity by HPLC

Pyrroloquinoline Quinone 

Hyphomicrobium sp. fermentation origin, CAS 122628-50-6; disodium salt form, >99% purity by HPLC; redox cofactor isolate

d-Alpha-Tocopherol (Vitamin E)

Non-GMO sunflower oil distillate, CAS 59-02-9; natural d-isomer form, >96% purity by HPLC

Full Ingredient List

[__]

Shellfish-free

Dairy-free

No binders

Vegan

Gluten-free

Cattle-free

No fillers

FDA-Approved labs

Poultry-free

Allergen-free

No preservatives

Third-party tested

Our Scientific and Clinical Advisory Board

Our Scientific and Clinical Advisory Board consist of scientist and clinicians across key fields, including longevity, microbiology, genomics, metabolomics, gastroenterology, immunology, nutrition, and canine clinical research.

Our Scientific and Clinical Advisory Board

Our Scientific and Clinical Advisory Board consist of scientists and clinicians across key fields, including longevity, microbiology, genomics, metabolomics, gastroenterology, immunology, nutrition, and canine clinical research. 

Katarina Lukman, DVM

Clinical Advisor

Marta Segui, DVM

Clinical Advisor

Michael Silber, DVM

Clinical Advisor

Nuno Revez, DVM

Scientific Advisor

Mikaela Alessandrini, DVM

Scientific Advisor

Questions?
We're here to help.

What benefits can I expect to see?

Most owners notice increased energy levels, joint health and coat in as little as 2 to 3 weeks. Other longer term benefits like cardiovascular health and gut immune function may take a little longer. As long as you continue with the daily intake, ESB-01 is doing its work inside to program, sustain, and enhance your dogs health.

How do I serve the ESB-01? 

Open 1 capsule and pour over breakfast every morning.

Why should my dog take the ESB-01?

Because the signs of aging you see: lower energy, stiffer joints, slower recovery, all trace back to one root cause — your dog's cells gradually losing the ability to repair themselves. 

 

ESB-01 works at that level, restoring what the body naturally loses over time and supporting the cellular systems that determine how your dog ages before the visible signs have a chance to compound.

Can it be taken with other supplements or medication?

ESB-01 may be layered or used alone. We've seen it used safely alongside antibiotics, prebiotics, probiotics, fish oils, and other supplements.

What does it taste like?

ESB-01 is flavorless to blend seamlessly into your dog’s regular meal.

Is it safe for dogs with sensitive stomachs?

Yes, it is made with human-grade ingredients and without fillers. In fact, the ESB-01 is generally easier on the stomach than many traditional supplements.

Is it safe for my dog's kidney and liver?

No adverse effects on the kidney or liver have been reported at the recommended daily serving. 

Michael Silber, DVM

Clinical Advisor

Katarina Lukman, DVM

Clinical Advisor

Marta Segui, DVM

Clinical Advisor

Nuno Revez, DVM

Scientific Advisor

Mikaela Alessandrini, DVM

Scientific Advisor

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